ATP is a biomarker for biomass and can be used as a valuable indicator for biofouling. Until now, ATP in seawater could not be measured using luciferin/luciferase reaction due to interference from salt. In this work we describe new methods to measure microbial ATP in seawater. The new method involves extraction of ATP directly from biomass in seawater, followed by detection of microbial ATP using novel reagents optimized for application in seawater. We show that the new ATP method is a promising tool for monitoring microbial growth in seawater, and can also be used to determine the concentration of Assimilable Organic Carbon (AOC) in seawater.
This presentation is available to AMTA Members only.
- Subhanjan /Almotasembellah Mondal / Sergio.G Abushaban / M. Nasir Salinas-Rodriguez / Said A. Mangal / Maria D. Goueli / Kennedy
- Promega Corporation / UNESCO-IHE Institute for Water Education
- AMTA/AWWA Membrane Technology Conference, Long Beach, CA
- AMTA/AWWA Membrane Technology Conference
- Membrane, Seawater, Ultrafiltration, Desalination, Reverse Osmosis, Fouling