This study examines the recovery of enteric adenovirus HAdV 40 by crossflow ultrafiltration from several water matrices (deionized water, tap water and surface water) and interprets recovery data by elucidating the physicochemical mechanisms that control virus adhesion on surfaces. In accordance with predictions from the extended Derjaguin-Landau-Verwey-Overbeek theory, pre-elution recovery of HAdV 40, from deionized water was higher with the PEM-coated membranes than with CS-blocked membranes. Eluent containing sodium polyphosphate and tween 80 effectively disrupt electrostatic and hydrophobic interactions between the virus and the membrane, leading post-elution recovery close to 100%. Eluent composition is the most important factor for high virus recovery from complex water matrices. addition of ethylenediamine tetraacetic acid (EDTA) to the eluent greatly improves the elution efficacy even when organic carbon concentration was high.
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- Hang Shi / Irene Xagoraraki / Kristin N. Parent / Merlin L. Bruening / Volodymyr V. Tarabara
- Michigan State University / University of Notre Dame
- AMTA/AWWA Membrane Technology Conference, Long Beach, CA
- AMTA/AWWA Membrane Technology Conference
- Membrane, Ultrafiltration, Concentrate, Design, Research